群馬大学医学部薬理学分野

群马大学医学院，药理学研究室（Department of Pharmacology, Gunma University Graduate School of Medicine）

大家好！感谢您访问我们的主页！新的药理学研究室成立于2020年10月1日。

在过去几十年中，神经精神障碍类疾病，包括精神分裂症、痴呆症和自闭症谱系障碍患者的数量迅速增加。我们致力于研究这些疾病的发病机制。这些患者大多存在突触传递问题。在复杂的神经元网络中，突触是传递信息的中继点，突触传递异常被认为是神经精神障碍类疾病的原因。为了在分子水平上探究疾病的发病机制，我们应用生化和超分辨荧光显微镜技术-受激发射损耗（stimulated emission depletion，STED）显微镜和随机光学重建显微镜(stochastic optical reconstruction microscopy，STORM)，研究相应疾病模型小鼠。(基于这些新的研究发现，我们正计划研发新的药物靶点，并开发新的神经精神障碍类疾病治疗方法)

群马大学位于日本的正中央，可以很方便的经东京到达。这所大学被美丽的大自然环绕着。透过研究室的窗户，我们就可以欣赏到赤城山、榛名山和妙义山的美妙景色。我非常期待看到他们如何在四季交替变化。群马大学作为一所以神经科学为研究特色的大学有着悠久的传统，在日本一直处于神经科学研究的领先地位。在日本，我们的大学是少数几所设备齐全、能够进行尖端神经科学研究的大学之一。

我们正在招收有科学抱负的本科生和研究生。为什么不加入我们的研究室呢？在我们研究室，本科生能够学习到当今前沿神经科学中非常有趣的知识；我们通过教学与培训使毕业研究生能够成为真正的专业科学家。学生在我们研究室的成就可能会被收编在教科书中，并改变科学的历史。只有在科学领域，你才能享受到这样令人兴奋的事情！

在以前工作的地方，德国马克斯·普朗克基础医学研究所，我一直在指导很多学生。他们有着不同的教育背景，分别来自日本、保加利亚、土耳其、波兰、台湾、印度、埃及、塞尔维亚和德国。与我在马克斯·普朗克同组毕业的博士们目前都在顶级研究机构和大学从事博士后工作或者担任研究团队负责人。基于个人的经历，我有能力和意愿指导来自世界各地的学生。我的梦想是将来从我群马大学团队中毕业的博士学生能够在世界各地从事他们的科学事业。

如果你有兴趣加入我的小组，或者如果你想了解更多的细节，请与我联系。我的电子邮件地址是kawabe@gunma-u.ac.jp。

Lab Members

Professor
Hiroshi Kawabe
I love doing good science and training students to be good scientists.

Associate Professor
Kenji Hanamura
I study the mechanism of neuronal development and function. My ultimate goals are understanding the pathophysiology of various neuropsychiatric disorders and developing therapeutic methods for the disorders.

Assistant Professor
Hiroyuki Yamazaki
I will do my best to contribute to the further development of science in Japan.

Assistant Professor
Noriko Koganezawa
I am very much interested in the mechanism of memory formation, especially at the synaptic level.
I love bench work and enjoy it every day to make a breakthrough in neuroscience.

Secretary
Tomoko Isono
I am organizing the lab so that lab members can concentrate on and enjoy their research.

Technical Assistant
Kazumi Kamiyama
I am doing my best to support daily experiments to develop science in the department.

Technical Assistant
Sachiko Tanaami
Lab works are a lot of fun, indeed. I am highly motivated to support research in the department.

Graduate Student
Mai Yamamura
Learning science in our department is a lot of fun. I proceed my project day by day to write manuscript in the next year.

Under Graduate Student
Banri Segawa
I will be a “two-way” professional; as a medical doctor and a neuroscientist!

對於學生

怎样加入药理学研究室呢？

有三种方法可以加入我们

如果你是群马大学昭和校区的本科生，可以上完课后来我们研究室看看。我们研究室已经有一名来自研究生-博士课程的学生在努力工作。
如果想在我们研究室读研究生，你可以参加硕士或博士课程的考试。我们非常欢迎来自各个国家的学生。
如果你来自公司或其他大学，想使用我们研究室的设备与我们共同合作研究，同样欢迎你。直接联系我就可以(kawabe@gunma-u.ac.jp)。

研究

我们研究什么？

1)超分辨显微镜的发展

正如您在我们主页的顶部所看到的，我们用STED显微镜检测到的结构与用标准共聚焦显微镜看到的结构完全不同。应用超分辨显微镜，我们将能够发现细胞或组织中未知的结构。为了获得更高的分辨率探索体内结构，我们正在努力提高我们的STED显微镜和STORM的分辨率。

2)了解神经精神障碍类疾病的潜在发病机制

最近很多证据表明，神经精神障碍类疾病是由遗传原因引起的。有些是单基因疾病，而另一些是寡基因疾病。在某些病例中，疾病的发展受到环境因素的影响。我们感兴趣的是参与蛋白质降解和调控细胞骨架重排的致病基因。我们已经建立了几个存在这种缺陷基因的突变小鼠系。我们将利用这些小鼠系研究精神和神经疾病潜在的分子机制。

3)通过蛋白质的翻译后修饰来研究神经细胞功能的调节机制

蛋白质是经过转录和随后的翻译合成的，表达的蛋白质通过与其他蛋白质结合或催化酶反应发挥作用。蛋白质的功能是通过翻译后修饰来调节，包括磷酸化和泛素化，这些修饰同样也会导致修饰蛋白的降解。我们一直致力于探究特定泛素化和细胞外磷酸化对神经细胞功能调节的作用。

我们的合作者

在国际上我们一直与下列世界不同国家和地区机构的同事合作。学生和博士后将有机会访问他们并开展实验。

德国

马克斯·普朗克基础医学研究所研究所、马克斯·普朗克生物物理化学研究所、柏林查理特大学、哥廷根大学、汉姆堡·埃彭多夫大学、吕贝克大学、莱比锡大学、乌尔姆大学、亚琛工业大学、马克斯·普朗克免疫生物学和表观遗传学研究所、莱布尼茨神经生物学研究所

瑞士

苏黎世联邦理工学院、洛桑大学

法国

巴黎-索邦大学

英国

邓迪大学

西班牙

巴塞罗那大学

比利时

列日大学

美国

哈佛大学、斯坦福大学、托马斯杰斐逊大学、马里兰大学、西北大学、斯克里普斯研究所、索尔克生物研究所

加拿大

多伦多大学、不列颠哥伦比亚大学

澳大利亚

阿德莱德大学

巴西

圣保罗大学

中国

南京大学、天津医科大学

新加坡

新加坡国立大学

Publication

Publication by Prof. Kawabe

Ambrozkiewicz MC*, Borisova E, Schwark M, Ripamonti S, Schaub T, Smorodchenko A, Weber AI, Rhee HJ, Altas B, Yilmaz R, Mueller S, Piepkorn L, Horan ST, Straussberg R, Zaqout S, Jahn O, Dere E, Rosário M, Boehm-Sturm P, Borck G, Willig K, Rhee JS, Tarabykin V, Kawabe H.* (2020) The murine ortholog of Kaufman oculocerebrofacial syndrome protein Ube3b regulates synapse number by ubiquitinating Ppp3cc. Molecular Psychiatry accepted. *=Corresponding authors
Hoshino S, Kobayashi M, Tagawa R, Konno R, Abe T, Furuya K, Miura K, Wakasawa H, Okita N, Sudo Y, Mizunoe Y, Nakagawa Y, Nakamura T, Kawabe H, Higami Y. (2020) WWP1 knockout mice have exacerbated obesity-related phenotype in white adipose tissue but improved whole body glucose metabolism. FEBS Open Bio 10:306-315.
Luo L, Ambrozkiewicz MC, Benseler F, Chen C, Dumontier E, Falkner S, Furlanis E, Gomez AM, Hoshina N, Huang WH, M Hutchison MA, Itoh-Maruoka Y, Lavery LA, Li W, Maruo T, Motohashi J, Pai ELL, Pelkey KA, Pereira A, Philips T, Sinclair JL, Stogsdill JA, Traunmüller L, Wang J, Wortel J, You W, Abumaria N, Beier KT, Brose N, Burgess HA, Cepko CL, Cloutier JF, Eroglu C, Goebbels S, Kaeser PS, Kay JN, Lu W, Luo L, Mandai K, McBain CJ, Nave KA, Prado MAM, Prado VF, Rothstein J, Rubenstein JLR, Saher G, Sakimura K, Sanes JR, Scheiffele P, Takai Y, Umemori H, Verhage M, Yuzaki M, Zoghbi HY, Kawabe H*, Craig AM*. (2020) Optimizing nervous system-specific gene targeting with Cre driver lines: prevalence of germline recombination and influencing factors. Neuron 106:1–29. *=Corresponding authors
Ambrozkiewicz MC*, Schwark M, Kishimoto-Suga M, Borisova E, Hori K, Salazar-Lázaro A, Rusanova A, Altas B, Piepkorn L, Bessa P, Schaub T, Zhang X, Rabe T, Ripamonti S, Rosário M, Akiyama H, Jahn O, Kobayashi T, Hoshino M, Tarabykin V, Kawabe H*. (2018) Polarity acquisition in cortical neurons is driven by synergistic action of Sox9-regulated Wwp1 and Wwp2 E3 ubiquitin ligases and intronic miR-140. Neuron 100:1097-1115. *=Corresponding authors
Ogawa M, Matsuda R, Tomokiyo M, Takada N, Yamamoto S, Shizukusihi S, Yamaji T, Yoshikawa Y, Yoshida M, Tanida I, Koike M, Murai M, Morita H, Takeyama H, Ryo A, Guan JL, Yamamoto M, Inoue JI, Yanagawa T, Fukuda M, Kawabe H, Ohnishi M. (2018) Molecular mechanisms of Streptococcus pneumoniae-targeted autophagy via pneumolysin, Golgi-resident Rab41, and Nedd4-1 mediated K63-linked　ubiquitination. Cellular Microbiology 20:e12846.
Bhouri M, Morishita W, Temkin P, Goswami D, Kawabe H, Brose N, Südhof TC, Craig AM, Siddiqui TJ, Malenka R. (2018) Deletion of LRRTM1 and LRRTM2 in adult mice impairs basal AMPA receptor transmission and LTP in hippocampal CA1 pyramidal neurons. Proc. Natl. Acad. Sci. USA. 115:E5382-E5389.
Pei G, Buijze H, Liu H, Moura-Alves P, Goosmann C, Brinkmann V, Kawabe H, Dorhoi A, Kaufmann SHE. (2017) The E3 ubiquitin ligase NEDD4 enhances killing of membrane-perturbing intracellular bacteria by promoting autophagy. Autophagy 13:2041-2055.
Henshall TL, Manning JA, Alfassy OS, Boase NA, Goel P, Kawabe H, Kumar S. (2017) Deletion of Nedd4-2 results in progressive kidney disease in mice. Cell Death Differ. 24:2150-2160.
Lee JH, Jeon SA, Kim BG, Takeda M, Cho JJ, Kim DI, Kawabe H, Cho JY. (2017) Nedd4 deficiency in vascular smooth muscle promotes vascular calcification by stabilizing pSmad1. J. Bone Miner. Res. 32:927-938.
Crosby JR, Zhao C, Jiang C, Bai D, Katz M, Greenlee S, Kawabe H, McCaleb M, Rotin D, Guo S, Monia BP. (2017) Inhaled ENaC antisense oligonucleotide ameliorates cystic fibrosis-like lung disease in mice. J. Cyst. Fibros. 16:671-680.
Zhou Z, Kawabe H, Suzuki A, Shinmyozu K, Saga Y. (2017) NEDD4 controls spermatogonial stem cell homeostasis and stress response by regulating messenger ribonucleoprotein complexes. Nat. Commun. 8:15662.
Sigler A, Oh WC, Imig C, Altas B, Kawabe H, Cooper BH, Kwon HB, Rhee JS, Brose N. (2017) Formation and maintenance of functional spines in the absence of presynaptic glutamate release. Neuron 94:304-311.
Kawabe H, Mitkovski M, Kaeser PS, Hirrlinger J, Opazo F, Nestvogel D, Kalla S, Fejtova A, Verrier SE, Bungers SR, Cooper BH, Varoqueaux F, Wang Y, Nehring RB, Gundelfinger ED, Rosenmund C, Rizzoli SO, Südhof TC, Rhee JS, Brose N. (2017) ELKS1 localizes the synaptic vesicle priming protein bMunc13-2 to a specific subset of active zones. J. Cell Biol. 216:143-161.
Jiang C, Kawabe H, Rotin D. (2017) The ubiquitin ligase Nedd4L regulates the Na/K/2Cl co-transporter NKCC1/SLC12A2 in the colon. J. Biol. Chem. 292:137-145.
Ripamonti S, Ambrozkiewicz MC, Guzzi F, Gravati M, Biella G, Bormuth I, Hammer M, Tuffy LP, Sigler A, Kawabe H, Nishimori K, Toselli M, Brose N, Parenti M, Rhee JS. (2017) Transient oxytocin signaling primes the development and function of excitatory hippocampal neurons. elife 6:22466.
Lu C, Thoeni C, Connor A, Kawabe H, Gallinger S, Rotin D. (2016) Intestinal knockout of Nedd4 enhances growth of Apcmin tumors. Oncogene 35: 5839- 5849.
Canal M, Martín-Flores N, Pérez-Sisqués L, Romaní-Aumedes J, Altas B, Man HY, Kawabe H, Alberch J, Malagelada C. (2016) Loss of NEDD4 contributes to RTP801 elevation and neuron toxicity：implications for Parkinson’s disease. Oncotarget 7:58813-58831.
Lipina TV, Prasad T, Yokomaku D, Luo L, Connor SA, Kawabe H, Wang YT, Brose N, Roder JC, Craig AM. (2016) Cognitive deficits in Calsyntenin-2-deficient mice associated with reduced GABAergic transmission. Neuropsychopharmacology 41:802-810.
Ott C, Martens H, Hassouna I, Oliveira B, Erck C, Zafeiriou MP, Peteri UK, Hesse D, Gerhart S, Altas B, Kolbow T, Stadler H, Kawabe H, Zimmermann WH, Nave KA, Schulz-Schaeffer W, Jahn O, Ehrenreich H. (2015) Widespread expression of erythropoietin receptor in brain and its induction by injury. Mol. Med. 21:803-815.
Hsia HE, Kumar R, Luca R, Takeda M, Courchet J, Nakashima J, Wu S, Goebbels S, An W, Eickholt B, Polleux F, Rotin D, Wu H, Rossner M, Bagni C, Rhee JS, Brose N, Kawabe H*. (2014) Ubiquitin E3 ligase Nedd4-1 acts as a downstream target of PI3K/PTEN-mTORC1 signaling to promote neurite growth. Proc. Natl. Acad. Sci. USA. 111:13205-13210. *=Corresponding author
Pettem KL, Yokomaku D, Luo L, Linhoff MW, Prasad T, Connor SA, Siddiqui TJ, Kawabe H, Chen F, Zhang L, Rudenko G, Wang YT, Brose N, Craig AM. (2013) The specific α-Neurexin interactor Calsyntenin-3 promotes excitatory and inhibitory synapse development. Neuron 80:113-128.
Siddiqui TJ, Tari PK, Conner SA, Zhang P, Dobie FA, She K, Kawabe H, Wang YT, Brose N, Craig AM. (2013) An LRRTM4-HSPG complex mediates excitatory synapse development on dentate gyrus granule cells. Neuron 79:680-695.
Nagpal P, Plant PJ, Correa J, Bain A, Takeda M, Kawabe H, Rotin D, Bain JR, Batt JA. (2012) The ubiquitin ligase Nedd4-1 participates in denervation-induced skeletal muscle atrophy in mice. PLOS One 7:e46427.
Cooper B, Hemmerlein M, Ammermüller J, Imig C, Reim K, Lipstein N, Kalla S, Kawabe H, Brose N, Brandstätter JH, Varoqueaux F. (2012) Munc13-independent vesicle priming at mouse photoreceptor ribbon synapses. J. Neurosci. 32:8040-8052.
Kimura T*, Kawabe H*, Jiang C, Zhang W, Xiang YY, Lu C, Salter MW, Brose N, Lu WY, Rotin D. (2011) Deletion of the ubiquitin ligase Nedd4L in lung epithelia causes cystic fibrosis-like disease. Proc. Natl. Acad. Sci. USA. 108:3216-3221. *=Equal contribution
Kawabe H*, Neeb A, Dimova K, Young SM Jr, Takeda M, Katsurabayashi S, Mitkovski M, Malakhova OA, Zhang DE, Umikawa M, Kariya K, Goebbels S, Nave KA, Rosenmund C, Jahn O, Rhee JS, Brose N*. (2010) Regulation of Rap2A by the ubiquitin ligase Nedd4-1 controls neurite development. Neuron 65:358-372. *=Corresponding authors
Fouladkou F, Lu C, Jiang C, Zhou L, She Y, Walls JR, Kawabe H, Brose N, Henkelman RM, Huang A, Bruneau BG, Rotin D. (2010) The ubiquitin ligase NEDD4-1 is required for heart development and is a suppressor of thrombospondin-1. J. Biol. Chem. 285:6770-6780.
Gómez-Varela D, Kohl T, Schmidt M, Rubio ME, Kawabe H, Nehring RB, Schäfer S, Stühmer W, Pardo LA. (2010) Characterization of Eag1 channel lateral mobility in rat hippocampal cultures by single-particle-tracking with quantum dots. PLOS One 5:e8858.
Trimbuch T, Beed P, Vogt J, Schuchmann S, Maier N, Kintscher M, Breustedt J, Schuelke M, Streu N, Kieselmann O, Brunk I, Laube G, Strauss U, Battefeld A, Wende H, Birchmeier C, Wiese S, Sendtner M, Kawabe H, Kishimoto-Suga M, Brose N, Baumgart J, Geist B, Aoki J, Savaskan NE, Bräuer AU, Chun J, Ninnemann O, Schmitz D, Nitsch R. (2009) Synaptic PRG-1 modulates excitatory transmission via lipid phosphate-mediated signaling. Cell 138:1222-1235.
Fouladkou F, Landry T, Kawabe H, Neeb A, Lu C, Brose N, Stambolic V, Rotin D. (2008) The ubiquitin ligase Nedd4-1 is dispensable for the regulation of PTEN stability and localization. Proc. Natl. Acad. Sci. USA. 105:8585-8590.
Dimova K*, Kawabe H*, Betz A, Brose N, Jahn O. (2006) Characterization of the Munc13-calmodulin interaction by photoaffinity labeling. Biochim. Biophys. Acta Mol. Cell Res. 1763:1256-1265. *=Equal contribution
Andrews-Zwilling YS, Kawabe H, Reim K, Varoqueaux F, Brose N. (2006) Binding to Rab3A-interacting molecule RIM regulates the presynaptic recruitment of Munc13-1 and ubMunc13-2. J. Biol. Chem. 281:19720-19731.
Kawabe H, Sakisaka T, Yasumi M, Shingai T, Izumi G, Nagano F, Deguchi-Tawarada M, Takeuchi M, Nakanishi H, Takai Y. (2003) A novel rabconnectin-3-binding protein that directly binds a GDP/GTP exchange protein for Rab3A small G protein implicated in Ca2+-dependent exocytosis of neurotransmitter. Genes Cells 8:537-546.
Nagano F*, Kawabe H*, Nakanishi H, Shinohara M, Deguchi-Tawarada M, Takeuchi M, Sasaki T, Takai Y. (2002) Rabconnectin-3, a novel protein that binds both GDP/GTP exchange protein and GTPase-activating protein for Rab3 small G protein family. J. Biol. Chem. 277:629-632. *=Equal contribution
Kawabe H, Nakanishi H, Asada M, Fukuhara A, Morimoto K, Takeuchi M, Takai Y. (2001) Pilt, a novel peripheral membrane protein at tight junctions in epithelial cells. J. Biol. Chem. 276:48350-48355.
Deguchi M, Iizuka T, Hata Y, Nishimura W, Hirao K, Yao I, Kawabe H, Takai Y. (2000) PAPIN. A novel multiple PSD-95/Dlg-A/ZO-1 protein interacting with neural plakophilin-related armadillo repeat protein/delta-catenin and p0071. J. Biol. Chem. 275:29875-29880.
Kikyo M, Matozaki T, Kodama A, Kawabe H, Nakanishi H, Takai Y. (2000) Cell-cell adhesion-mediated tyrosine phosphorylation of nectin-2delta, an immunoglobulin-like cell adhesion molecule at adherens junctions. Oncogene 19:4022-4028.
Yao I, Ohtsuka T, Kawabe H, Matsuura Y, Takai Y, Hata Y. (2000) Association of membrane-associated guanylate kinase-interacting protein-1 with Raf-1. Biochem. Biophys. Res. Commun. 270:538-542.
Hirao K, Hata Y, Deguchi M, Yao I, Ogura M, Rokukawa C, Kawabe H, Mizoguchi A, Takai Y. (2000) Association of synapse-associated protein 90/ postsynaptic density-95-associated protein (SAPAP) with neurofilaments. Genes Cells 5:203-210.
Hirao K, Hata Y, Yao I, Deguchi M, Kawabe H, Mizoguchi A, Takai Y. (2000) Three isoforms of synaptic scaffolding molecule and their characterization: multimerization between the isoforms and their interaction with N-methyl-D-aspartate receptors and SAP90/PSD-95-associated protein. J. Biol. Chem. 275:2966-2972.
Kawabe H, Hata Y, Takeuchi M, Ide N, Mizoguchi A, Takai Y. (1999) nArgBP2, a novel neural member of ponsin/ArgBP2/vinexin family that interacts with synapse-associated protein 90/postsynaptic density-95-associated protein (SAPAP). J. Biol. Chem. 274:30914-30918.
Shimizu K, Kawabe H, Minami S, Honda T, Takaishi K, Shirataki H, Takai Y. (1996) SMAP, an Smg GDS-associating protein having arm repeats and phosphorylated by Src tyrosine kinase. J. Biol. Chem. 271:27013-27017.

Publication by the former research group of the Department of Neurobiology and Behavior

Yin XL, Jia QF, Zhang GY, Zhang JP, Shirao T, Jiang CX, Yin XY, Liu YS, Chen P, Gu XC, Qian ZK, Yin GZ, Sen Xia H, Hui L. (2019) Association between decreased serum TBIL concentration and immediate memory impairment in schizophrenia patients. Sci. Rep. 9:1622.
Miki D, Kobayashi Y, Okada T, Miyamoto T, Takei N, Sekino Y, Koganezawa N, Shirao T, Saito Y. (2019) Characterization of functional primary cilia in human induced pluripotent stem cell-derived neurons. Neurochem. Res. 44:1736-1744.
Mitsuoka T, Hanamura K, Koganezawa N, Kikura-Hanajiri R, Sekino Y, Shirao T. (2019) Assessment of NMDA receptor inhibition of phencyclidine analogues using a high-throughput drebrin immunocytochemical assay. J. Pharmacol. Toxicol. Methods. 106583
Hanamura K, Koganezawa N, Kamiyama K, Tanaka N, Oka T, Yamamura M, Sekino Y, Shirao T. (2019) High-content imaging analysis for detecting the loss of drebrin clusters along dendrites in cultured hippocampal neurons. J. Pharmacol. Toxicol. Methods. 106607.
Puspitasari A, Yamazaki H, Kawamura H, Nakano T, Takahashi A, Shirao T, Held KD. (2019) X-irradiation of developing hippocampal neurons causes changes in neuron population phenotypes, dendritic morphology and synaptic protein expression in surviving neurons at maturity. Neurosci. Res. S0168-0102: 30320-7.
Miao S, Koganezawa N, Hanamura K, Puspitasari A, Shirao T. (2018) N-methyl-D-aspartate receptor mediates X-irradiation-induced drebrin decrease in hippocampus. Kitakanto Medical Journal 68: 111-115
Hanamura K, Kamata Y, Yamazaki H, Kojima N, Shirao T. (2018) Isoform-dependent regulation of drebrin dynamics in dendritic spines. Neuroscience 379: 67–76
Yamazaki H, Sasagawa Y, Yamamoto H, Bito H, Shirao T. (2018) CaMKIIβ is localized in dendritic spines as both drebrin-dependent and drebrin-independent pools. J. Neurochem. 146:145-159.
Yasuda H, Kojima N, Yamazaki H, Hanamura K, Sakimura K, Shirao T. (2018) Drebrin isoforms critically regulate NMDAR- and mGluR-dependent LTD induction. Front. Cell. Neurosci. 12:330.
小金澤紀子、花村健次、白尾智明 (2017) 「ヒトiPS細胞由来神経細胞を用いた医薬品評価系の現状について」日本薬理学雑誌 149: 104-109
Shirao T, Hanamura K, Koganezawa N, Ishizuka Y, Yamazaki H, Sekino Y. (2017) The role of drebrin in neurons. J. Neurochem. 141:819-834.
Koganezawa N, Hanamura K, Sekino Y, Shirao T. (2017) The role of drebrin in dendritic spines. Mol. Cell. Neurosci. 84:85-92.
Li B, Ding S, Feng N, Mooney N, Ooi YS, Renf L, Diep J, Kelly MR, Yasukawa LL, Patton JT, Yamazaki H, Shirao T, Jackson PK, Greenberg HB. (2017) Drebrin restricts rotavirus entry by inhibiting dynamin-mediated endocytosis. Proc. Natl. Acad. Sci. USA. 114:E3642-E3651.
Kajita Y, Kojima N, Koganezawa N, Yamazaki H, Sakimura K, Shirao T. (2017) Drebrin E regulates neuroblast proliferation and chain migration in the adult brain. Eur. J. Neurosci. 46:2214-2228.
Hanamura K, Washburn HR, Sheffler-Collins SI, Xia NL, Henderson N, Tillu DV, Hassler S, Spellman DS, Zhang G, Neubert TA, Price TJ, Dalva MB. (2017) Extracellular phosphorylation of a receptor tyrosine kinase controls synaptic localization of NMDA receptors and regulates pathological pain. PLoS Biol. 15:e2002457.
Shirao T, Sekino Y. (Eds.) (2017) Drebrin. (Advances in Experimental Medicine and Biology, vol 1006) Tokyo, Springer
Sekino Y, Koganezawa N, Mizui T, Shirao T. (2017) Role of Drebrin in Synaptic Plasticity. Adv. Exp. Med. Biol. 1006:183-201.
Yamazaki H, Shirao T. (2017) Homer, Spikar, and Other Drebrin-Binding Proteins in the Brain. Adv. Exp. Med. Biol. 1006:249-268.
Shirao T, Koganezawa N, Yamazaki H, Hanamura K, Imamura K. (2017) Localization of Drebrin: Light Microscopy Study. Adv. Exp. Med. Biol. 1006:105-118.
Shirao T, Sekino Y. (2017) General Introduction to Drebrin. Adv. Exp. Med. Biol. 1006:3-22.
Ishizuka Y, Hanamura K. (2017) Drebrin in Alzheimer’s Disease. Adv. Exp. Med. Biol. 1006:203-223.
Hanamura K. (2017) Drebrin in Neuronal Migration and Axonal Growth. Adv. Exp. Med. Biol. 1006:141-155.
Puspitasari A, Koganezawa N, Ishizuka Y, Kojima N, Tanaka N, Nakano T, Shirao T. (2016) X Irradiation Induces Acute Cognitive Decline via Transient Synaptic
Dysfunction. Radiat. Res. 185:423-30.
Fujieda T, Koganezawa N, Ide Y, Shirao T, Sekino Y. (2015) An Inhibitory Pathway Controlling the Gating Mechanism of the Mouse Lateral Amygdala Revealed by Voltage-Sensitive Dye Imaging. Neuroscience Letters 590:126-131.
Xu S, Buraschi S, Morcavallo A, Genua M, Shirao T, Peiper SC, Gomella LG, Birbe R, Belfiore A, Iozzo RV, Morrione A. (2015) A novel role for drebrin in regulating progranulin bioactivity in urothelial cancer. Oncotarget 6:10825-39.
Ishizuka Y, Shimizu H, Takagi E, Kato M, Yamagata H, Mikuni M, Shirao T. (2014) Histone deacetylase mediates the decrease in drebrin cluster density induced by amyloid beta oligomers. Neurochem. Int. 76:114-121.
Tanabe K, Yamazaki H, Inaguma Y, Asada A, Kimura T, Takahashi J, Taoka M, Ohshima T, Furuichi T, Isobe T, Nagata K, Shirao T, Hisanaga S. (2014) Phosphorylation of drebrin by cyclin-dependent kinase 5 and its role in neuronal migration. PLOS One 9:e92291.
Ishikawa M, Shiota J, Ishibashi1 Y, Hakamata T, Shoji S, Fukuchi M, Masaaki Tsuda M, Shirao T, Sekino Y, Baraban JM, Tabuchi A. (2014) Cellular localization and dendritic function of rat isoforms of the SRF coactivator MKL1 in cortical neurons. NeuroReport 25:585-592.
Mizui T，Sekino Y, Yamazaki H, Ishizuka Y, Takahashi H, Kojima N, Kojima M, Shirao T. (2014) Myosin Ⅱ ATPase activity mediates the long-term potentiation-induced exodus of stable F-actin bound by drebrin A from dendritic spines. PLOS One 9: e85367.
Yamazaki H, Kojima N, Kato K, Hirose H, Iwasaki T, Mizui T, Takahashi H, Hanamura K, Roppongi RT, Koibuchi N, Sekino Y, Mori N, Shirao T. (2013) Spikar, a novel drebrin-binding protein, regulates the formation and stabilization of dendritic spines. J. Neurochem. 128:507-522.
白尾智明、山崎博幸 (2013)「樹状突起棘（スパイン）のアクチンフィラメントと微小管」 Clinical Neuroscience 31: 1302-1395.
Ishikawa M, Shiota J, Ishibashi Y, Hakamata T, Shoji S, Fukuchi M, Tsuda M, Shirao T, Sekino Y, Ohtsuka T, Baraban JM, Tabuchi A. (2013) Identification, expression and characterization of rat isoforms of the SRF coactivator MKL1. FEBS Open Bio. 3:387-393.
Ishizuka Y, Kakiya N, Witters LA, Oshiro N, Shirao T, Nawa H, Takei N. (2013) AMP-activated protein kinase (AMPK) counteractsbrain-derived neurotrophic factor (BDNF)-induced mammalian target of rapamycin complex 1 (mTORC1) signaling in neurons. J. Neurochem. 127:66-77.
Shirao T, Gonzalez-Billault C. (2013) Actin filaments and microtubules in dendritic spines. J. Neurochem. 126:155-164.
Roppongi RT, Kojima N, Hanamura K, Yamazaki H, Shirao T. (2013) Selective reduction of drebrin and actin in dendritic spines of hippocampal neurons by activation of 5-HT2A receptors. Neurosci. Lett. 547:76-81.
Shirai K, Mizui T, Suzuki Y, Okamoto M, Hanamura K, Yoshida Y, Hino M, Noda S, AL-Jahdari WS, Chakravarti A, Shirao T, Nakano T. (2012) X-Irradiation Changes Dendritic Spine Morphology and Density through Reduction of Cytoskeletal Proteins in Mature Neurons. Radiation Research 179:630-636.
Canto CB, Koganezawa N, Beed P, Moser EI, Witter MP. (2012) All layers of medial entorhinal cortex receive presubicular and parasubicular inputs. J. Neurosci. 32: 17620-17631.
Kato K, Shirao T, Yamazaki H, Imamura K, Sekino Y. (2012) Regulation of AMPA receptor recruitment by the actin binding protein drebrin in cultured hippocampal neurons. J. Neurosci. Neuroeng. 1: 153-160.
Tanaka K, Sato K, Yoshida T, Fukuda T, Hanamura K, Kojima N, Shirao T, Yanagawa T, and Watanabe H. (2011) Evidence for cell density affecting C2C12 myogenesis: possible regulation of myogenesis by cell-cell communication. Muscle and Nerve 44:968-977.
Kobayashi-Yamazaki C, Shirao T, Sasagawa Y, Maruyama Y, Akita H, Saji M, Sekino Y. (2011) Lesions of the supramammillary nucleus decrease self-grooming behavior of rats placed in an open field The Kitakanto Medical Jorunal 61:287-292.
Han W, Takamatsu Y, Yamamoto H, Kasai S, Endo S, Shirao T, Kojima N, Ikeda K. (2011) Inhibitory role of inducible cAMP early repressor (ICER) in methamphetamine-induced locomotor sensitization. PLOS One 6:e21637.
Okamoto T, Endo S, Shirao T, Nagao S. (2011) Role of Cerebellar Cortical Protein Synthesis in Transfer of Memory Trace of Cerebellum-Dependent Motor Learning. J. Neurosci. 31:8958-66.
Kambe T, Motoi Y, Inoue R, Kojima N, Tada N, Kimura T, Sahara N, Yamashita S, Mizoroki T, Takashima A, Shimada K, Ishiguro K, Mizuma H, Onoe H, Mizuno Y, Hattori N. (2011) Differential regional distribution of phosphorylated tau and synapse loss in the nucleus accumbens in tauopathy model mice. Neurobiol. Dis. 42:404-414.
Kaminuma T, Suzuki Y, Shirai K, Mizui T, Noda S, Yosida Y, Funayama T, Takahasi T, Kobayashi Y, Shirao T, Nakano T. (2010) Effectiveness of carbon-ion beams for apoptosis induction in rat primary immature hippocampal neurons. J. Rad. Res. 51:627-631.
Hanamura K, Mizui T, Kakizaki T, Roppongi TR, Yamazaki H, Yanagawa Y, Shirao T (2010) Low accumulation of drebrin at glutamatergic postsynaptic sites on GABAergic neurons. Neuroscience 169:1489-1500.
Perez-Martinez M, Gordon-Alonso M, Cabrero JR, Barrero-Villar M, Rey M, Mittelbrunn M, Lamana A, Morlino G, Calabia C, Yamazaki H, Shirao T, Vazquez J, Gonzalez-Amaro R, Veiga E, Sanchez-Madrid F. (2010) F-actin-binding protein drebrin regulates CXCR4 recruitment to the immune synapse. J. Cell Sci. 123:1160-1170.
Mercer JC, Mottram LF Qi Q, Lee YC, Bruce D, Iyer A, Yamazaki H, Shirao T, Choe MH, Peterson BR, August A. (2010) Chemico-Genetic Identification of Drebrin as a Regulator of Calcium Responses. Int. J. Biochem. Cell Biol. 42:337-345.
Kojima N, Hanamura K, Yamazaki H, Ikeda T, Itohara S, Shirao T. (2010) Genetic disruption of the alternative splicing of drebrin gene impairs context-dependent fear learning in adulthood. Neuroscience 165: 138-150.
Okamoto M, Suzuki Y, Shirai K, Mizui T, Yoshida Y, Noda S, Al-Jahdari WS, Shirao T, Nakano T. (2009) Effect of Irradiation on the Development of Immature Hippocampal Neurons In Vitro. Radiat. Res. 172:718-724.
Aoki C, Kojima N, Sabaliauskas N, Shah L, Oakford J, Ahmed T, Yamazaki H, Hanamura K, Shirao T. (2009) Drebrin A Knock-Out Eliminates the Rapid Form of Homeostatic Synaptic Plasticity at Excitatory Synapses of Intact Adult Cerebral Cortex. J. Comp. Neurol. 517:105-121.
Ito M, Shirao T, Doya K, Sekino Y. (2009) Three-dimensional distribution of Fos-positive neurons in the supramammillary nucleus of the rat exposed to novel environment. Neurosci. Res. 64:397-402.
Mizui T, Kojima N, Yamazaki H, Katayama M, Hanamura K, Shirao T. (2009) Drebrin E is involved in the mechanism regulating axonal growth through actin-myosin interactions. J. Neurochem. 109:611-622.
Takahashi T, Yamazaki H, Hanamura K, Sekino Y, Shirao T. (2009) AMPA receptor inhibition causes abnormal dendritic spines by destabilizing drebrin. J. Cell. Sci. 122:1211-1229.
Ivanov A, Esclapez M, Pellegrino1 C, Shirao T, Ferhat L. (2009) Drebrin A regulates the dendritic spine plasticity and synaptic function in cultured hippocampal neurons. J. Cell. Sci. 122: 524-534.
Kojima N, Borlikova G, Sakamoto T, Yamada K, Ikeda T, Itohara S, Niki H, Endo S. (2008) Inducible cAMP early repressor acts as a negative regulator for kindling epileptogenesis and long-term fear memory. J. Neurosci. 28:6459-6472.
Song M, Kojima N, Hanamura K, Sekino Y, Inoue KH, Mikuni M, Shirao T. (2008) Expression of drebrin E in migrating neuroblasts in adult rat brain: coincidence between drebrin E disappearance from cell body and cessation of migration. Neuroscience 152:670-682.
Kobayashi C, Aoki C, Kojima N, Yamazaki H, Shirao T. (2007) Drebrin A content correlates with spine head size in the adult mouse cerebral cortex. J. Comp. Neurol. 503:618-26.
Sekino Y, Kojima N, Shirao T. (2007) Role of actin cytoskeleton in dendritic spine morphogenesis. Neurochem. Int. 51:92-104.
Kato K, Sekino Y, Takahashi H, Yasuda H, Shirao T. (2007) Increase of AMPA receptors-mediated miniature EPSC amplitude after chronic NMDA receptor blockade in cultured hippocampal neurons. Neurosci. Lett. 418: 4-8.
Kojima N, Shirao T. (2007) Synaptic dysfunction and disruption of the postsynaptic drebrin-actin complex: the study of neurological disorders accompanied by cognitive deficits. Neurosci. Res. 58: 1-5.
Majoul I, Shirao T, Sekino Y, Duden R. (2007) Many faces of Drebrin: from building dendritic spines and stabilizing gap junctions to shaping neurite-like cell processes. Histochem. Cell. Biol. 127: 355-361.
Shirai K, Mizui T, Suzuki Y, Kobayashi Y, Nakano T, Shirao T. (2006) Differential effects of x-irradiation on immature and mature hippocampal neurons in vitro. Neurosci. Lett. 399: 57-60.
Chang EH, Savage MJ, Flood DG, Thomas JM, Levy RB, Mahadomrongkul V, Shirao T, Aoki C, Huerta PT. (2006) AMPA receptor downscaling at the onset of Alzheimer’s pathology in double knock-in mice. Proc. Natl. Acad. Sci. USA. 103: 3410-3415.
Fujisawa S, Shirao T, Aoki C. (2006) In vivo, competitive blockade of NMDA receptors induces rapid shape change of post-synaptic spines and F-actin reorganization within dendritic spines of adult rat cortex. Neuroscience 140:1177-1187.
Takahashi H, Mizui T, Shirao T. (2006) Downregulation of drebrin A expression suppresses synaptic targeting of NMDA receptors in developing hippocampal neurons. J. Neuochem. 97(s1):110-115.
Sekino Y, Tanaka S, Hanamura K, Yamazaki H, Sasagawa Y, Xue Y, Hayashi K, Shirao T. (2006) Activation of N-methyl-D-aspartate receptor induces a shift of drebrin distribution: disappearance from dendritic spines and appearance in dendritic shafts. Mol. Cell. Neurosci. 31: 493-504.
Mahadomrongkul V, Huerta PT, Shirao T, Aoki C. (2005) Stability of the distribution of spines containing drebrin A in the sensory cortex layer I of mice expressing mutated APP and PS1 genes. Brain Res. 1064: 66-74.
Mizui T, Takahashi H, Sekino Y, Shirao T. (2005) Overexpression of drebrin A in immature neurons induces the accumulation of F-actin and PSD-95 into dendritic filopodia, and the formation of large abnormal protrusions. Mol. Cell. Neurosci. 30: 149-157.
Aoki C, Sekino Y, Hanamura K, Fujisawa S, Mahadomrongkul V, Ren Y, Shirao T. (2005) Drebrin A is a Postsynaptic Protein that Localizes in vivo to the Submembranous Surface of Dendritic Sites Forming Excitatory Synapses. J. Comp. Neurol. 483:383-402.

Link to the former department of neurobiology and behavior：http://neuro.dept.med.gunma-u.ac.jp/?page_id=96&lan=en